Sequence polymorphism of the waxy gene in waxy maize accessions and characterization of a new waxy allele

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Nucleotide sequence of rice waxy gene.

Rice waxy gene codes for the starch granule-bound starch synthase (EC 2.4.1.11) (1). To characterize its molecular structure, a rice {Oryza sativa subsp. japonica Hengfeng cultivar) genomic library was constructed in XEMBL-3 vector. Two overlapping genomic clones containing waxy gene sequence were screened and identified from this library by using maize waxy gene DNA as probe (kindly provided b...

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A Rare Carbohydrate in Waxy Maize.

The dextrins, which occur as successive steps in the transition from glucose to starch and from starch to glucose, are so rarely detected in plant tissues and so ephemeral in existence, that their occurrence in stable form in matured or dormant tissues has seldom been reported. It has been the occasion of no little interest, therefore, to find that the sole carbohydrate in the endosperm of “wax...

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An Enzyme Difference Associated with the Waxy Gene in Maize.

The amylase of waxy and non-waxy seeds. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 573 The amylase of waxy and non-waxy pollen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 577 Resultsof tests with S, W, and S, W, pollen extracts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 579 Results of tests wit...

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Molecular and Chemical Characterization of a New Waxy Allele in Barley (Hordeum vulgare L.)

Cereal Chem. 91(5):438–444 Barley m38 mutant was selected for its high level of mixed-linkage (1,3),(1,4)-β-D-glucan (MLG) in the grain. This elevated level of MLG was found to be associated with decreased amylose accumulation as well as other chemical composition alterations. Molecular characterization results revealed m38 as a new allele of the Waxy gene, encoding an endosperm-specific granul...

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Waxy and non-waxy barley cultivars exhibit differences in the targeting and catalytic activity of GBSS1a

Amylose synthesis is strictly associated with activity of granule-bound starch synthase (GBSS) enzymes. Among several crops there are cultivars containing starch types with either little or no amylose known as near-waxy or waxy. This (near) amylose-free phenotype is associated with a single locus (waxy) which has been mapped to GBSS-type genes in different crops. Most waxy varieties are a resul...

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ژورنال

عنوان ژورنال: Scientific Reports

سال: 2020

ISSN: 2045-2322

DOI: 10.1038/s41598-020-72764-3